首先恭賀東北大學武老師使用上海恒遠生物自主品牌ELISA試劑盒在《ACS Nano》（美國化學學會）期刊上發(fā)表的SCI文獻——Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive and High-Precision Screening of Early Urinary Diseases順利通過，IF影響因子高達15.88分。同時恭賀武老師喜提上海恒遠生物贈送的獎學金，正所謂雙喜臨門呀！

優(yōu)秀的文獻值得記錄與分享，也希望對正在做實驗和寫論文的科研小伙伴們有所幫助，通過文獻我們可以更好的了解到產品，如果您也使用恒遠品牌的ELIA試劑盒發(fā)表了SCI文獻，歡迎您積極的參與我們的“發(fā)lun文贏獎學金"活動，小編等著你們的投稿哦，下一個領獎學金的說不定就是你！

【文獻標題】Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive and High-Precision Screening of Early Urinary Diseases

【作者單位】東北大學（Northeastern University）

【文獻中引用產品】

人CD63分子（CD63）ELISA試劑盒

【關鍵詞】uEVs ,DNA machine, fluorescence detection ,machine learning, urinary diseases

【影響因子(IF)】15.88

【出版期刊】《ACS Nano》

【產品原文引用】

Materials. 

All the oligonucleotides were synthesized and purified by Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, China), and their sequences are listed in Table S1. All oligonucleotide stock solutions were prepared by dissolving DNA strands in Tris-HCl buffer (10 mmol L-1 Tris, 10 mmol L-1 MgCl2, pH 8.0) and stored at -20 °C refrigerator before use. All the DNA strands were annealed at 95 °C for 5 min and slowly cooled down to room temperature to obtain stable structures. 1-Ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC) and N?hydroxysuccinimide (NHS) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Sodium hydroxide (NaOH), Na2HPO4·12H2O, NaH2PO4·2H2O, AgNO3 and KCl were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). 2-(N Morpholino) ethanesulfonic acid (MES), Gel-Red, loading buffer and 5×TBE buffer were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). BCA Protein Quantification Kit was provided by Beyotime Biotechnology (Shanghai, China). Dulbecco’s modified eagle medium (DMEM) was acquired from Aladdin Reagent Co. Ltd. (Shanghai, China). Carboxyl magnetic nanoparticles (COOH-MNPs, 200 nm) was purchased from PuriMag Biotech Co. Ltd. (Xiamen, China). Exo III, phi29 DNA polymerase were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). T4 DNA ligase and deoxynucleotides (dNTPs) were purchased from Takara Biotechnology Co. (Dalian, China). Thioflavine T (ThT) was obtained from Yuanye Bio-Technology Co. Ltd. (Shanghai, China). Anti?CD63 rabbit monoclonal antibody(ab193349) was purchased from Abcam Co. Ltd. (Shang, China). Anti-CD9 rabbit monoclonal antibody (20597-1-ap), anti-TSG101 rabbit monoclonal antibody (28283-1-AP) and β-actin monoclonal antibody (66009-1-Ig) were purchased from Proteintech Group Co. Ltd. (Chicago, USA). ELISA (human CD63) kit was purchased from Shanghai Hengyuan Biological Technology (Shanghai, China). All chemicals were analytical grade and directly used without further purification. 

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