【文獻標(biāo)題】Prenatal nicotine exposure induces poor articular cartilage quality in female adult offspring fed a high-fat diet and the intrauterine programming mechanisms

【作者】Kai Tie，Yang Tan，Yu Deng，et.al

【作者單位】武漢大學(xué)中南醫(yī)院（Zhongnan Hospital of Wuhan University）

【文獻中引用產(chǎn)品】

試劑-藏紅o試劑(CAS. No. 477-73-6)

試劑-固綠FCF試劑(CAS. No. 2353-45-9)

【關(guān)鍵詞】Prenatal nicotine exposure，Intrauterine programming，Cholesterol efflux dysfunction，Cholesterol deposition，Poor articular cartilage quality

【DOI】dx.doi.org/10.1016/j.reprotox.2015.12.010

【影響因子(IF)】5.60

【出版期刊】《Reproductive Toxicology》

【產(chǎn)品原文引用】

Nicotine (CAS. No. 54-11-5) was purchased from Sigma–Aldrich Co., Ltd.(St. Louis, MO, USA). Safranin O (CAS. No. 477-73-6) and fast green (CAS. No. 2353-45-9) were obtained from Hengyuan Biotech Co., Ltd. (Shanghai, China). Isoflurane was purchased from Baxter Healthcare Co. (Deerfield, IL, USA). Rat corticosterone (CORT)ELISA kits were obtained from R&D Systems, Inc. (Minneapolis,MN, USA). Rat IGF1 ELISA kits were purchased from RD Systems,Inc. (Minneapolis, MN, USA). Total cholesterol (TCH), low-density lipoproteincholesterol(LDL-C) andhigh-density lipoproteincholesterol (HDL-C) assay kits were purchased from Sangon Biotech Co.,Ltd. (Shanghai, China). Tissue TCH assay kits were purchased from Applygen Tech, Inc. (Beijing, China). Reverse transcription and quantitative PCR (Q-PCR) kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). DAB staining kits were obtained from GeneTech Company, Ltd. (Shanghai, China). GeXP multiplex gene expression analysis kits were purchased from BeckmanCoulter Inc. (Fullerton, CA, USA). The oligonucleotide primers for rat Q-PCR genes (PAGE purification) and GeXP multiplex gene expression analysis (HPLC purification) were synthesized by Sangon Biotech Co., Ltd.(Shanghai, China). Polyclonal antibodies for the 1 chain of type II collagen gene (Col2A1), IGF1, PPAR, LXR and ABCA1were obtained from SantaCruz Biotechnology, Inc. (Santa Cruz, CA, USA). Other chemicals and agents were of analytical grade.

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